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. Author manuscript; available in PMC: 2013 Sep 12.
Published in final edited form as: J Am Chem Soc. 2012 Aug 30;134(36):14753–14755. doi: 10.1021/ja307453v

Figure 2.

Figure 2

2D 15N-1H HETCOR spectra of membrane-bound His37-labeled M2TM at (a) pH 6.0, (b) pH 4.5, and (c) pH 8.5 at 245 K (left) and 296 K (right). The main charged states of the M2TM channel at each pH are indicated. The 1H dimension of the spectrum was measured with 1H homonuclear decoupling to eliminate spin diffusion effects. Assignments are shown in red for the neutral τ tautomer, black for the neutral π tautomer, and green for cationic His37.