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Unassembled Fet3-3HAp is incorporated into the ER-derived vesicles. In vitro budding assay was performed with microsomes prepared from Δftr1 Δpep4 (SMY531) expressing FET3-3HA. Fifteen percent of total reaction and budded vesicle fractions were analyzed by immunoblotting with anti-HA, anti-Sec22p, and anti-Sec61p antibodies. Sec22p and Sec61p represent a positive and negative control, respectively.
