Skip to main content
. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Org Chem. 2013 Feb 13;78(6):2213–2247. doi: 10.1021/jo3026077

Table 3.

Determination of IC50 Values of Compound 3 against Human Cancer Cells (nM).

DU145 HL60 KG1a MDA-MD-435
36.4 ± 2.9 7.4 ± 0.6 3.3 ± 0.9 94.5 ± 8.0

Cell viability was performed using MTS assays as described in Methods. IC50 values of compound 3 were determined in human DU145 prostate cancer, MDA-MB-435 breast cancer, KG1a AML and HL60 AML cells. Cells were seeded in 96- well plates (5000/well for DU145 and MDA-MB-435, 10000/well for KG1a and HL60), incubated overnight at 37°C in 5% (v/v) CO2 and exposed to compound 3 in a dose-dependent manner for 72 h. Absorbance was measured at 490 nm using an automated ELISA plate reader. Each experiment was performed in quadruplicate. Data are mean ± SD.