Fig. 1.

Comparative 2D-DIGE proteomics profiles of human lymph and plasma. A) 2D-DIGE image of proteins expressed in plasma (Cy3 green fluorescence) and lymph (Cy5 red fluorescence). One out of three independent experiments is shown. (B) 2D-DIGE of differentially expressed lymph and plasma proteins. The green spots represent proteins up-regulated in plasma while red spots indicate proteins up-regulated in lymph. Circles indicate proteins having at least a 3.0 fold change in fluorescence expression index (defined as the ratio lymph/plasma). These proteins were excised from the gel and subjected to nanoLC–ESI–MS/MS analysis. Nineteen protein spots were selected with a lymph/plasma ratio above 4.2 and are presented in Table 1 (11 spots are displayed in Fig. 1B and 8 spots are displayed in Supplementary Fig. 1). Proteins with at least a 3.0 fold change in the lymph/plasma fluorescence index are presented in Supplementary Table 1. (C) 3D view of individual spots of proteins significantly up-regulated (≥+8.0 fold change) in the lymph as compared with the plasma following analysis using DeCyder software. Each protein is also reported in Table 1. The up-regulation of tetranectin (CLEC3B) expression in the lymph as compared with plasma was validated by Western analysis (Fig. 5). BAI-1 associated protein 2 was not yet validated by Western blot but its major unique peptide MS/MS fragmentation profile is included in Supplementary Fig. 3.