Figure 1.

ago1⁺ and dcr1⁺ are both required for normal cytokinesis. All strains were cultured at 30°C in YE to mid-log phase (OD₅₉₅ = 0.6-0.9), stained with 4,6-diamidino-2-phenylindole and placed onto microscope slides coated with poly l-lysine (5 mg/ml). Samples were examined by fluorescence and differential interference contrast microscopy. Representative fields were photographed and scored for nuclei or septation. For each sample, at least 500 cells were scored. (A) Quantitation of binucleated cells in parental strain TV294, Δago1 (TV292), Δdcr1 (TV293), and Δrdp1 (TV296) strains. (B) Quantitation of septation indices of TV294, Δago1 (TV292), Δdcr1 (TV293), and Δrdp1 (TV296). (C) Quantitation of septation indices in FY254 and Δago1 (JC254K) strains transformed with vector (pREP3X), pREP3X +ago1⁺, or pREP3X +hAgo2.