Figure 5.

ago1⁺ functions downstream of dcr1⁺ to regulate cell cycle events. Yeast strains were transformed with plasmids encoding Ago1, HA-Dcr1, or vector alone. (A) Yeast strains grown in EMM-leu to log phase were examined by phase contrast microscopy, and septated cells were counted and expressed as a percentage of total cells. (B) Yeast strains were streaked onto EMM lacking leucine (EMM) in the presence or absence of 7.5 mM HU (+HU) and were then cultured at 30°C for 3 to 5 d.