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Ectopic overexpression of Ago1 does not correct the chromosome segregation defect of dcr1 nulls. Yeast strains were transformed with plasmids encoding Ago1, HA-Dcr1 or vector alone. Strains were streaked onto EMM lacking leucine in the presence (+TBZ) or absence (EMM) of 10 μg/ml TBZ and were then cultured at 30°C for 3 to 5 d.
