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. 2013 Apr 2;110(16):6560–6565. doi: 10.1073/pnas.1219704110

Fig. 3.

Fig. 3.

Inactivation of the M. tuberculosis Rv1002c gene impairs Apa protein O-mannosylation and impacts on Mtb growth. (A) Impact of the Rv1002c inactivation on the Mtb ΔRv1002c mutant growth in Middlebrook 7H9 broth with Albumin-Dextrose-Catalase or dextrose alone (Inset). (B) Growth impairment of the mutant on solid Middlebrook 7H11 agar medium. Serial dilution of cultures containing 108 bacteria per milliliter were plated and colony sizes were compared after 4 wk of growth at 37 °C. (C, Right) Relative abundances of the Apa peptides detected in the culture filtrates of wild-type (Wt), ΔRv1002c (Mt), and complemented ΔRv1002c:Rv1002c (Cp) mutant (sum of the abundances are set at 100% for each strain). (Left) Weighted deviation from the mean relative abundance of each peptide in the three strains. (D) LC-MS reconstructed ion chromatograms of the P278–321, P285–321, and P287–321 Apa C-terminal peptides and their monoglycosylated forms (*) detected in the different Mtb strains; (reconstructed ion chromatograms parameters as in Fig. 2).