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Identification of acetylated peptides and proteins by LC-MS/MS. (A) Western blot analysis of glutamate dehydrogenase (GDH) and SIRT3 in mitochondrial lysates from WT and SIRT3−/− mouse livers. (B) Overlap of identified acetylated proteins and peptides from WT and SIRT3−/− mice. (C) Distribution of acK sites identified per protein. (D) Pathway analysis of the mitochondrial acetylome with the number of proteins identified per pathway indicated.
