Fig. 4.

Pathway and evolutionary analysis of acetylation sites and consensus motifs. (A) Pathway analysis of the mitochondrial acetylome altered in SIRT3^−/− mice with the number of proteins identified per pathway. (B) Heat map depicting the conservation index of SIRT3 substrates (twofold increase) across seven vertebrate species with percent conservation calculated for all acK sites identified and SIRT3 substrates. Conservation index of all nonregulated sites is available in Fig. S3. (C) Percentage of acK residues identified in mouse mutated to arginine (P = 0.01) or glutamine (P = 0.04) across the seven species in Fig. 3B (P values calculated via χ² test). (D) Consensus sequence logos plot for acetylation sites ± six amino acids from the lysine of all acK sites identified, (E) from the identified lysine residues significantly increased in SIRT3^−/− mice, and (F) for highly conserved acetylation sites significantly increased in SIRT3^−/− mice.