Fig. 3.

(A) GFP fluorescence in live spermatozoa imaged directly under epifluorescence illumination (Top) and in the fixed spermatozoa processed with anti-GFP antibody (Middle and Bottom); spermatozoa from a wild-type boar (WT) served as a negative control. (B) GFP fluorescence is strongest in the nuclei of a live day 6 blastocyst created by in vitro fertilization of wild-type oocytes with spermatozoa from a GFP boar (homozygous son of the PSMA1-GFP line founder). (Inset) Onset of PSMA1-GFP gene expression at the four-cell stage, coinciding with the major MZT of transcription control.