Figure 6.

The recombinant SAA proteins activate neutrophils with different potency. (A) Neutrophils purified from buffy coats were incubated for 20 h with addition of medium, or rSAA1 (filled squares; n = 3) at the indicated concentrations. Apoptosis was thereafter evaluated by flow cytometry on the basis of Annexin V positive events. Shown for rSAA1 is mean (±SEM) and one representative experiment with rSAAh (open squares) is shown for comparison. Statistical analysis was performed using repeated measurement ANOVA followed by Dunnett‘s multiple comparison test (**p < 0.01). (B) Extracellular release of ROS from TNF-α primed neutrophils after addition (arrow) of indicated concentrations of rSAA1 or rSAAh. Representative curves (upper) are shown with arrows indicating the addition of SAA, as well as mean peak values (±SEM; lower) from three independent experiments.