Figure 3. Insulin does not activate the fluorescence enhancement for LS456 at 700 nm in Akt1-deficient mammary tumor cells.

(a) The fluorescence intensity (FI) of LS456 in mammary epithelial cells decreased in the 800-nm channel and increased in the 700 nm channel, which did not occur in Akt1-deficient cells. (b, c) Quantitative analysis of the FI in WT and Akt1-deficient cells. Fluorescence images in cells are superimposed on differential interference contrast images All figures are at the same scale bars: 20 μm; error bars are reported as mean ± standard deviation (N = 8).