Fig. 5.

Validation of miR-203 targets by 3′UTR luciferase assay. (A) Twelve out of 15 targets are repressed by miR-203 when their 3′UTR is tested in the luciferase assay. The positive control (2×miR-203) bears two perfectly matched sites for full-length miR-203 and shows ∼95% repression. (B) When the predicted miR-203 target sites are specifically mutated, seven out of eight targets are derepressed in the presence of miR-203 in the 3′UTR luciferase assay. For all assays, n=3, ^*P≤0.05, ^**P≤0.01, ^***P≤0.001, N.S., not significant. Data are mean±s.e.m.