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. 2013 May 1;140(9):1924–1935. doi: 10.1242/dev.090209

Fig. 3.

Fig. 3.

Ectopic overexpression of HDG2 induces some epidermal reporters. (A,B) Histological cross-sections of emerging rosette leaves from 10-day-old wild-type (wt: A) and HDG2-OX seedlings expressing AtML1pro::GUS (B). Ectopic GUS expression (asterisks) can be seen in B. Scale bars: 100 μm. (C-F) In situ hybridization analysis of FDH expression in 10-day-old wild-type (C,D) and HDG2-OX (E,F) seedlings treated with HDG2 antisense (C,E) or sense (D,F) probes. Scale bars: 40 μm. (G) At5g17710pro::nuc-3xVENUS in wild-type rosette leaf abaxial epidermis showing specific signals in large pavement cells. Scale bar: 50 μm. (H,I) Confocal optical sections (xy, xz and yz planes) of 10-day-old HDG2-OX cotyledon expressing At5g17710pro::nuc-3xVenus, without (H) or with (I) HDG2 induction. Asterisk, GFP in subepidermal mesophyll nucleus. Arrowheads, GFP epidermal nuclei (co-stained with propidium iodide). Scale bars: 50 μm.