Fig. 6.
Deletion of laminins retards vascular growth. (A-D) Vascular growth was assayed by CD31 expression at P5 in retinal wholemounts. (E) Vascular growth was charted for the indicated ages and genotypes (n=3 or 4). Error bars represent s.e.m. No outgrowth of endothelial cells were seen in the Lamb2:c3-/- mice from P1 to P5. (F-I) Vascular growth assayed at P15 from indicated genotypes. Vascular growth recovers by P15 in the Lamc3-/- (G) but not the other Lamb2 mutants; vessels in I are persistent hyaloid vessels. (J) Tip cells were counted in a 150 μm2 region in each quadrant of the P5 retina (n=4). Error bars represent s.d. ***P≤0.0002, **P≤0.001.