Effect of duration of diabetes on mtDNA copy number. Mitochondria DNA copy number was assessed by real-time PCR in 10 ng retinal gDNA using primers for (a) COII and (b) Cytb. The expression was normalized by that of nuclear gene β-actin. Values are presented as mean ± SD from 5 to 8 rats in each group. Nor, normal; 15d, rats diabetic for 15 days; and 2mo, 6mo, and 12mo, rats diabetic for 2 months, 6 months, and 12 months, respectively. *P <0.05 compared to normal.