Table 1.
Secretion values and general characteristics for secretory cargo
| Type | Protein | AA (with SP) | Cys | N-CHO | Pro | Secretion time (approx.) | Level | References |
|---|---|---|---|---|---|---|---|---|
| Soluble | Insulin | 86 (110) | 6 | 0 | 6 | 35 min | ND | (Straub and Sharp 2002) |
| RNase (bovine) | 124 (150) | 8 | 1 | 4 | 35 min | 59% | (Geiger et al. 2011) | |
| RNase (human) | 127 (156) | 8 | 3 | 7 | 27 min | 73% | (Geiger et al. 2011) | |
| MD2 | 142 (160) | 7 | 2 | 7 | 2 h | 90% | (Visintin et al. 2001) | |
| Cp SFV | 148 (172) | 0 | 0 | 7 | 40 min | 72% | (Thor et al. 2009) | |
| α2-HS-glycoprotein | 348 (367) | 14 | 2 | 39 | 25 min | 70% | (Rutkevich et al. 2010) | |
| A1AT | 394 (410) | 1 | 3 | 17 | 44 min | 40% | (Lodish and Kong 1984; Rutkevich et al. 2010) | |
| IgG2b | 457 (476) | 13 | 2 | 37 | 100 min | 60% | (Hendershot et al. 1987) | |
| gp120 LAI | 486 (516) | 18 | 23 | 22 | 2 h | 40% | (Land et al. 2003) | |
| HA anchor- A/Japan/305/1957 (H2N2) | 509 (524) | 12 | 4 | 18 | 1 h | >90% | (Singh et al. 1990) | |
| Alkaline-phosphatase | 513 (535) | 5 | 2 | 31 | 30 h | ND | (Aldag et al. 2011) | |
| HA anchor- A/Aichi/2/1968 (H3N2) | 513 (531) | 12 | 7 | 20 | 1 h | >90% | (Singh et al. 1990) | |
| Albumin | 584 (609) | 35 | 0 | 24 | 45 min | >80% | (Rutkevich et al. 2010) | |
| α-Fetoprotein | 590 (609) | 32 | 1 | 21 | 45 min | 100% | (Rutkevich et al. 2010) | |
| Transferrin | 679 (698) | 40 | 2 | 32 | 1 h | 80% | (Rutkevich et al. 2010) | |
| Factor V | 2196 (2224) | 19 | 26 | 151 | 3 h | ∼100% | (Duga et al. 2003) | |
| Thyroglobulin | 2749 (2768) | 122 | 17 | 173 | 1 h | 50% | (Kim and Arvan 1991) | |
| Single pass | NA A/WSN/33 (H1N1) | 453 | 19 | 4 | 21 | 30–60 min | ND | (Hogue and Nayak 1992; Popp et al. 2012) |
| VSVG | 495 (511) | 12 | 2 | 27 | 30–60a min | 80%-100%a | (Doms et al. 1988) | |
| Tyrosinase | 511 (529) | 15 | 6 | 33 | 30 min | 38%b | (Popescu et al. 2005) | |
| HA | 550 (566) | 12 | 7 | 20 | 20 min | 100% | (Braakman et al. 1991) | |
| Transferrin R | 734 (760) | 6 | 3 | 31 | 3 h | 50% | (Lodish et al. 1983) | |
| TLR2 | 748 (766) | 13 | 4 | 25 | 3 h | 50% | (Lin et al. 2000) | |
| gp160 Env BH8 | 821 (851) | 20 | 28 | 29 | 2 h | ∼35% | (Earl et al. 1991) | |
| gp160 Env LAI | 831 (861) | 20 | 29 | 29 | 4 h/20 ha | 30%/30% | (Bird et al. 1990; Land et al. 2003) | |
| LDL R | 836 (860) | 61 | 3 | 39 | 1 h | 50%c | (Jansens et al. 2002) | |
| EGF R | 1210 (1186) | 50 | 12 | 75 | 1.5 h | 50% | (Gamou et al. 1989) | |
| Multi-pass | Aquaporin2 | 271 | 1 (4) | 1 | 14 | ∼1.5 h | ∼50% | (Hendriks et al. 2004) |
| hACH R | 482 | 8 | 1 | 31 | 1.5 h | 30%, | (Merlie and Lindstrom 1983) | |
| Shaker K+ channel | 656 | 2 | 2 | 32 | 45 min | ND | (Schulteis et al. 1995; Khanna et al. 2001) | |
| hCFTR in BHK cells | 1454 | 0 (18) | 2 | 45 | ∼2 h | 50% | (Mendes et al. 2003) | |
| hCFTR in HeLa cells | 1454 | 0 (18) | 2 | 45 | ∼1 h | 80% | (Hoelen et al. 2010) | |
| hCFTR in HEK293 cells | 1454 | 0 (18) | 2 | 45 | ∼1 h | 50% | (Zhang et al. 2002) |
The number of amino acids in a protein is designated without and with (parentheses) signal sequences included. N-CHO indicates the predicted number of N-linked glycans. The number of Cys in the luminal ectodomain of transmembrane proteins is indicated in parentheses. Secretion times and levels are approximate values.aDepending on expression system and cells.bReaches melanosomes rather than the plasma membrane.cT1/2 of glycan maturation endo H resistance.