TABLE 1.
Antibodies for immunohistochemical analysis
| Antibody | Company | Catalog No. | Type | Clone | Dilution | Antigen retrieval | Positive staining pattern |
|---|---|---|---|---|---|---|---|
| VEGF | DAKO | M7273 | Monoclonal mouse | VG1 | 1/6400 | Citrate buffer | Cytoplasmic |
| EGFR | DAKO | M3563 | Monoclonal mouse | H11 | 1/400 | Citrate buffer | Cytoplasmic |
| p53 | DAKO | M7001 | Monoclonal mouse | DO-7 | 1/50 | Citrate buffer | Nuclear |
| GLUT-1 | DAKO | A3536 | Polyclonal rabbit | Not available | 1/300 | Citrate buffer | Cytoplasmic membrane |
| Cyclin D1 | DAKO | M7155 | Monoclonal mouse | DCS-6 | 1/50 | Citrate buffer | Nuclear |
VEGF, Vascular endothelial growth factor; EGFR, epidermal growth factor receptor; GLUT-1, glucose transporter 1.