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. 2013 Mar 28;451(Pt 2):185–194. doi: 10.1042/BJ20130026

Figure 2. USP52 potentiates the hypoxic response.

Figure 2

(A) Depletion of USP52 using siRNA si1 or si3 in U2OS cells reduces the levels of HIF1A, but not HIF1B, protein as assessed by Western blot analysis. HIF1A transcriptional targets GLUT1 and LDHA also showed decreased protein expression. Tubulin was used as a loading control. Molecular masses are indicated in kDa. (B) Real-time RT–PCR analysis shows that the expression of HIF1A targets CA9, PHD2 and VEGF are all induced in U2OS cells in response to hypoxia. USP52 knockdown reduces the ability of cells to increase expression of all HIF1A target genes. Levels were normalized to β-actin. Results are means±S.E.M.