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. 2013 Feb 26;41(8):e92. doi: 10.1093/nar/gkt115

Figure 3.

Figure 3.

Expression of stably integrated multigene transposon vectors in NIH 3T3 cells. (a) Diagram of vectors containing one, three or five fluorescent proteins along with the puromycin-tk (puro-tk) fusion. (b) Representative fluorescence photomicrographs and QRT-PCR analysis of stably integrated insulated and uninsulated multigene vectors. Expression levels are normalized to uninsulated vector gene expression for direct comparison of insulated and uninsulated vectors. P-values were calculated using a two-tailed unpaired t-test (P > 0.0001***, P > 0.001**, P > 0.01*).