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. 2013 Feb 26;41(8):e92. doi: 10.1093/nar/gkt115

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Transposition efficiency of multigene vectors. (a,b) Bar graph demonstrating the number of puromycin-resistant stable integration clones generated in HEK293T cells after transfection of transposase and insulated or uninsulated transposon vectors. (c) Representative sequencing results of PB splinkerette analysis of stable transposon integration cell lines with each vector tested. All cloned insertion sites contained inverted terminal repeat sequence, canonical TTAA insertion site sequence and adjacent genomic sequence.