Figure 2.

Csm2’s physical interaction by yeast two-hybrid with Rad51 is mediated by Rad55–Rad57. (A) Csm2 or Psy3 cloned into the pGBD plasmid (containing a GAL4-binding domain) was assayed for interaction with Rad55, Rad57 or Rad51, which were individually cloned into the pGAD (containing a GAL4-activating domain), by yeast two-hybrid. Growth on minimal medium lacking histidine indicates a yeast two-hybrid interaction, as HIS3 is the downstream reporter gene activated by interaction between the queried plasmids (interaction). Equal cell plating was determined by growth on minimal media lacking leucine and tryptophan, which selects for the two plasmids (loading control). (B) Csm2 and Rad51 or Csm2 and Rad55 yeast two-hybrid interactions were analysed in strains where RAD55 or RAD51 were, respectively, disrupted as described in (A).