Figure 3.

Csm2 is in the same epistasis group as Rad55 and regulates Rad55 recruitment to DNA damage sites. (A) WT, csm2Δ, rad55Δ and rad55Δ csm2Δ cells were 5-fold serially diluted onto yeast peptone dextrose (YPD) medium or YPD medium containing 0.006% MMS or exposed to 70 Gy IR and incubated at 23°C or 30°C for 2 days. (B) and (C) YFP-Rad55 expressing strains were analysed for the percentage of cells with a nuclear Rad55 focus before (untreated) or 20, 60, 120, and 240 min after IR (40 Gy). In part (B) images of Rad55 are shown, and a fluorescent Rad55 focus is indicated with a white arrowhead. Each experiment was done in triplicate with 300–500 cells analysed with standard errors plotted.