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Overexpression of Rad55–Rad57 or Rad51 does not suppress the MMS sensitivity of csm2Δ cells at 23°C. WT, csm2Δ and rad55Δ cells were either co-transformed with a Rad55 and Rad57 plasmid, a Rad51 plasmid or their respective empty vectors. Cells were grown to early log phase in minimal medium with selection for the plasmids and then 5-fold serially diluted onto YPD or YPD with 0.006% MMS. After 3 or 4 days of growth at 23°C, the plates were photographed.
