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. 2013 Mar 4;41(8):4525–4534. doi: 10.1093/nar/gkt138

Table 1.

Sequences of the DNA substrates used in Figure 1B, Figure 1C, and Supplemental Figure S1

DNA substrate Sequence
Single stranded 3′-GACGCTCGAGCTTAAGTGACCTCACTGGAG-5′
dsDNA 5′-CTGCGAGCTCGAATTCACTGGAGTGACCTC-3′
3′-GACGCTCGAGCTTAAGTGACCTCACTGGAG-5′
5′-overhang 5′-TCAAAGTCACGACCTAGACACTGCGAGCTCGAATTCACTGGAGTGACCTC-3′
3′-GACGCTCGAGCTTAAGTGACCTCACTGGAG-5′
3′-overhang 5′-CTGCGAGCTCGAATTCACTGGAGTGACCTC-3′
3′-AGTTTCAGTGCTGGATCTGTGACGCTCGAGCTTAAGTGACCTCACTGGAG-5′
Forka 5′-TTTTTTTTTTTTTTTTTTTTCTTGACAAGCTTGCGCACT-3′
3′-TTTTTTTTTTTTTTTTCACGGAACTGTTCGAACGCGTGA-5′

aThe fluorescein-labelled fork is identical to the fork substrate with a single-fluorescein molecule attached to the 3′-end of the top strand.