. 2013 Mar 4;41(8):4525–4534. doi: 10.1093/nar/gkt138

Table 1.

Sequences of the DNA substrates used in Figure 1B, Figure 1C, and Supplemental Figure S1

DNA substrate	Sequence
Single stranded	`3′-GACGCTCGAGCTTAAGTGACCTCACTGGAG-5′`
dsDNA	`5′-CTGCGAGCTCGAATTCACTGGAGTGACCTC-3′`
dsDNA	`3′-GACGCTCGAGCTTAAGTGACCTCACTGGAG-5′`
5′-overhang	`5′-TCAAAGTCACGACCTAGACACTGCGAGCTCGAATTCACTGGAGTGACCTC-3′`
5′-overhang	`3′-GACGCTCGAGCTTAAGTGACCTCACTGGAG-5′`
3′-overhang	`5′-CTGCGAGCTCGAATTCACTGGAGTGACCTC-3′`
3′-overhang	`3′-AGTTTCAGTGCTGGATCTGTGACGCTCGAGCTTAAGTGACCTCACTGGAG-5′`
Fork^a	`5′-TTTTTTTTTTTTTTTTTTTTCTTGACAAGCTTGCGCACT-3′`
Fork^a	`3′-TTTTTTTTTTTTTTTTCACGGAACTGTTCGAACGCGTGA-5′`

^aThe fluorescein-labelled fork is identical to the fork substrate with a single-fluorescein molecule attached to the 3′-end of the top strand.