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. 2013 Mar 12;41(8):4699–4708. doi: 10.1093/nar/gkt152

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Expression of an artificial miRNA targeting RRP44A leads to enhanced S-PTGS. (A) RNA gel blot analyses of three different Hc1 plant lines expressing the artificial RRP44A miRNA amiR-RRP44A. Small RNA gel blots were hybridized with a GUS DNA probe or an oligonucleotide antisense to the amiR. U6 served as a loading control for small RNA. (B) Reverse transcriptase-PCR of RRP44A and RRP44B transcripts in the corresponding Hc1/amiR-RRP44A and control Hc1 seedlings. EF1alpha was used as an amplification control. Normalized values of RRP44A and RRP44B mRNA to EF1 alpha mRNA (with Hc1 levels set at 1.0) are indicated. (C) The percentage of silenced plants determined by GUS quantitative protein assays in the indicated mutant and control lines. The number of plants analysed is indicated above each bar.