Figure 1.

Sequence and degradation of tRNAs in HeLa cells with or without heat stress. (A) Cloverleaf structures of our selected tRNAs are shown. The complimentary region to the DNA probe is illustrated, and the sequence of the DNA probe is given in Supplementary information. Northern blot analysis of tRNAs degradation at 37°C (B) and 43°C (C). 7.5 µg total RNA was loaded and blotted. Selected tRNAs were iMet (red), eMet (black), leucine (yellow), lysine (blue), proline (green). 5S rRNA transcribed by RNA polymerase III was used as a control. tRNAs and 5S rRNA were detected by a specific probe. Data represent the mean±SD for three independent experiments.