Figure 5.

Ser2448 phosphorylation level of mTOR. (A) HeLa cells were treated in the absence or presence of rapamycin for 30 min. Phosphorylation level of mTOR at Ser2448 was evaluated in the absence (right) or presence (left) of rapamycin by western blot analysis. Phospho-mTOR was normalized according to the expression level of mTOR. (B) Ser2448 phosphorylation of mTOR was detected under heat stress by western blotting analysis. mTOR was used as a control. (C) Circles, cells heat treated in the absence of rapamycin. Squares, cells heat treated in the presence of rapamycin. The ratio of phospho-mTOR was calculated as 1.0 for none treated zero time point. The expression of mTOR was used as a control. (D) Phosphorylation of mTOR under heat shock in the presence of rapamycin was detected.