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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2012 Jun 29;11(9):1852–1862. doi: 10.1158/1535-7163.MCT-12-0181

Figure 2.

Figure 2

CDODA-Me-dependent regulation of Sp transcription factors. Downregulation of Sp proteins by western blot (A) or immunostaining (B). Cells were treated with CDODA-Me for the indicated times and Sp1, Sp3 and Sp4 were analyzed by western blots or Immunostaining (Sp1 only) as described in the Materials and Methods. (C) Decreased Sp-regulated genes. Cells were treated as described in (A) and Sp-regulated genes were analyzed by western blots. (D) Sp1 knockdown alters cell cycle progression. Cells were transfected with siSp1 oligonucleotide and after 48 hr, cells were treated with 2.5 μM CDODA-Me for 24 hr and analyzed by FACS as described in the Materials and Methods. Results in (D) are expressed as means ± SE for three replicate determinations and significant (p < 0.05) increases (*) or decreases (**) compared to control non-specific oligonucleotide (CT) are indicated.