Table 3.
Effect of NaHS on RPMCs and ripples in the presence of lidocaine and carbachol
| RPMCs (proximal) | Control | Lidocaine (100 μM) | Serosal NaHS | |
|---|---|---|---|---|
| + Carbachol (10 μM) | 0.3 mM | 1 mM | ||
| Frequency (cpm) | 1.3 ± 0.1 | 1.8 ± 0.2a | 0.0 ± 0.0c | 0.0 ± 0.0c |
| Amplitude (mm) | 2.9 ± 0.4 | 3.8 ± 0.2 | 0.0 ± 0.0c | 0.0 ± 0.0c |
| Duration (s) | 35.6 ± 2.6 | 26.3 ± 1.0a | 0.0 ± 0.0c | 0.0 ± 0.0c |
| Propagation (cm min−1) | 5.2 ± 0.5 | 8.9 ± 1.0 | 0.0 ± 0.0c | 0.0 ± 0.0c |
| RPMCs (mid) | Control | Lidocaine (100 μM) | Serosal NaHS | |
|---|---|---|---|---|
| + Carbachol (10 μM) | 0.3 mM | 1 mM | ||
| Frequency (cpm) | 0.8 ± 0.2 | 1.0 ± 0.2 | 0.8 ± 0.5 | 0.3 ± 0.3 |
| Amplitude (mm) | 4.2 ± 0.5 | 4.6 ± 0.6 | 2.2 ± 1.3a | 0.1 ± 0.1b |
| Duration (s) | 37.1 ± 8.8 | 29.9 ± 0.6 | 15.3 ± 9.4 | 6.2 ± 6.2 |
| Propagation (cm min−1) | 25.5 ± 4.0 | 10.4 ± 1.6 | 5.0 ± 3.2 | 3.6 ± 3.6a |
| Ripples | Control | Lidocaine (100 μM) | Serosal NaHS | |
|---|---|---|---|---|
| + Carbachol (10 μM) | 0.3 mM | 1 mM | ||
| Frequency (cpm) | 11.3 ± 0.2 | 10.1 ± 1.0 | 8.2 ± 0.4 | 9.5 ± 0.9 |
| Amplitude (mm) | 0.7 ± 0.1 | 0.8 ± 0.1 | 1.6 ± 0.1a | 1.1 ± 0.4 |
| Duration (s) | 4.9 ± 0.1 | 4.9 ± 0.3 | 7.0 ± 0.3b | 5.8 ± 0.6 |
| Presence (cm)* | 3.4 ± 0.4 | 5.4 ± 0.8 | 6.3 ± 0.8 | 7.3 ± 0.7 |
| Propagation (cm min−1) | ||||
| Aboral (anterograde) | 6.0 ± 0.5 | 6.5 ± 1.1 | 7.1 ± 0.8 | 7.1 ± 0.8 |
| Oral (retrograde) | 5.6 ± 0.7 | 7.8 ± 0.7a | 7.2 ± 0.7 | 8.5 ± 1.5 |
Values are means ± SEM. n = 4.
a
P < 0.05;
b
P < 0.01;
c
P < 0.001, significant difference from control or lidocaine + carbachol respectively; paired Student's t-test (lidocaine + carbachol vs. control) and one-way ANOVA, followed by Bonferroni's multiple comparison test (NaHS vs. Lidocaine + carbachol).
*
Distance in which ripples were observed from the proximal edge of the colon.