Figure 2.

AMG 181 binds to both low- and high-affinity conformations of α₄β₇: human PBMCs were stained with 1 μg·mL⁻¹ of biotinylated MAdCAM-Fc (A, B) or biotinylated AMG 181 (C, D) in the absence (A, C) or presence (B, D) of 1 mM Mn²⁺ as indicated. MAdCAM-Fc or AMG 181 binding to the human CD4+CD45RA-memory T cells was measured by FACS analysis. X-axis represents fluorescence intensity of staining with biotinylated MAdCAM-Fc (upper panels) or biotinylated AMG 181 (lower panel) plus streptavidin-PE. Y-axis is the cell count.