Figure 1.

Inhibitory effect of cannabichromene on nitrite levels in the cell medium of murine peritoneal macrophages incubated with lipopolysaccharide (LPS, 1 μg·mL⁻¹) for 18 h. Cannabichromene (CBC, 0.001–1 μM) was added to the cell media 30 min before LPS challenge (i.e. 18.5 h before nitrites assay). Results are mean ± SEM of six experiments (in triplicates). ^#P < 0.001 versus control; *P < 0.05 and ***P < 0.001 versus LPS alone. The insert (on top of the figure) shows the effect of CBC (expressed as percentage of inhibition of the corresponding control values, with the difference between LPS and control considered as 100%) when given 30 min before LPS (CBC before LPS) or 15 h after LPS (CBC after LPS). No statistically significant difference was observed between the two concentration–response curves reported in the insert.