Figure 2.

Inducible nitric oxide synthase (iNOS) (A, B) and cyclooxygenase-2 (COX-2) (C, D) mRNA and protein levels in cell lysates from macrophages incubated or not with lipopolysaccharide (LPS, 1 μg·mL⁻¹) for 18 h. mRNA expression was evaluated by RT-PCR. The expression levels, normalized with respect to the reference genes, were scaled to the expression value of the control, considered as 1. The means of the quantitative-cycles (Cq) for the control were: 26.00 and 25.58 for iNOS and COX-2 respectively. The reaction background was N/A (see text) at 40 reaction cycles. Protein expression was evaluated by Western blot analysis. Cannabichromene (CBC, 1 μM) was added to the cell media 30 min before LPS challenge. ^#P < 0.001 versus control (n = 4–5 experiments).