Figure 4.

Effect of cannabichromene (CBC, 1 μM) alone and in presence of the cannabinoid CB₁ receptor antagonists rimonabant (0.1 μM) (A) and AM251 (1 μM) (B) as well as in the presence of the cannabinoid CB₂ receptor antagonist SR144528 (0.1 μM) (C) on nitrite levels in the cell medium of murine peritoneal macrophages incubated with lipopolysaccharide (LPS, 1 μg·mL⁻¹) for 18 h. The antagonists were added to the cell media 30 min before CBC exposure. LPS (1 μg·mL⁻¹for 18 h) was incubated 30 min after CBC. Results are means ± SEM of three experiments (in triplicates). ^#P < 0.001 versus control; *P < 0.05, **P < 0.01 and ***P < 0.001 versus LPS; ^°P < 0.05 versus LPS + CBC.