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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Neurobiol Aging. 2013 Jan 9;34(6):1581–1588. doi: 10.1016/j.neurobiolaging.2012.12.005

Fig. 5.

Fig. 5

PGC-1α expression promotes BACE1 degradation. (A) Primary hippocampal-cortical neurons derived from Tg2576 embryos at 14 days in vitro were infected by adenoviral-GFP PGC-1α, scramble PGC-1α-shRNA, or PGC-1α-shRNA, respectively, 72 hours after infection, cell lysates were collected and analyzed via Western blot using anti-BACE1 antibodies. BACE1 levels were quantified and normalized against the level of β-tubulin and plotted as percentage of CTL. Data are expressed as mean±standard error of the mean (n=5). * p<0.05 compared with CTL group. Inset represents PGC-1α immunoreactive signals. (B) The degradation of BACE1 caused by PGC-1α expression on was blocked by lactacystin treatment (5 µM). Data are expressed as mean ± standard error of the mean (n = 5). * p < 0.05 compared with CTL group. Inset represents BACE1 immunoreactive signals. Abbreviations: BACE1, β-secretase; CTL, control; GFP, green fluorescent protein; PGC-1α, peroxisome proliferator-activated receptor-γ coactivator 1α; shRNA, small hairpin RNA.