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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Hypertension. 2012 Sep 4;60(4):1006–1015. doi: 10.1161/HYPERTENSIONAHA.112.199661

Figure 1.

Figure 1

α2δ-1 and CaV1.2α1 subunit mRNA and protein are elevated in hypertension. A, Representative gel (of 3 experiments) illustrating RT-PCR amplification of transcripts for α2δ-1 through -4 in isolated arterial myocytes and whole brain of 12 week old WKY and SHR rats. B, Mean quantitative PCR data for α2δ-1 and CaV1.2α1 mRNA in 6 (n=8 for each) and 12 (n=6 for each) week old SHR rat arteries normalized to Rps5 and then to age-matched WKY controls. C, Exemplar Western blot illustrating α2δ-1 and CaV1.2α1 protein from 6 and 12 week old WKY and SHR rat whole arterial lysate. Blots were physically cut at 75 kDa to allow probing for actin and α2δ-1/CaV1.2α1. D, Mean data illustrating that α2δ-1 and CaV1.2α1 proteins are elevated in hypertension. * indicates P<0.05 when compared with the respective mRNA/protein at 6 weeks, # indicates P<0.05 when compared with CaV1.2α1 mRNA/protein at 12 weeks (n=8 for each protein at each age).