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. Author manuscript; available in PMC: 2013 Apr 22.
Published in final edited form as: Dev Dyn. 2012 Jun 23;241(8):1274–1288. doi: 10.1002/dvdy.23817

Figure 2. Slit molecules over-expression impairs neural crest cell migration.

Figure 2

Chicken embryos HH14-15 were electroporated with control GFP (A, C, E) or mSlit1 (B, F), hSlit2 (D) plasmids and incubated for 24 (A, B arrows point to forelimb area) or 48 (C–F arrows point to hindlimb area) hpe. Cells were visualized with anti-GFP or anti-myc for Slit1. Neural crest cells expressing Slit1 or Slit2 did not migrate as far as in control embryos (arrows in A–H), and Slit electroporated cells looked rounder and less dispersed than control GFP. Sections through a 48 hpe embryo at hindlimb level showed that a larger number of control electroporated cells reached the dorsal aorta (arrow in E) compared with mSlit1 expressing cells (arrow in F). Cultures of electroporated neural tubes (bright with DAPI) showed that mSlit1-expressing cells did not migrate as far as control GFP cells (arrows in G, H). Cross sections were counter-stained with HNK1 (red in E–F).