Figure 2.
Characterization of TMPRSS2 and ERG rearrangement patterns in prostate cancer cell lines determined by the 4-color FISH assay. (A and B) Metaphase FISH and karyogram images from fusion-negative LNCaP; (C and D) Metaphase FISH and karyogram images of fusion-positive VCap. White arrows indicate normal FISH signals, represented as clusters of the 4 probes. Empty arrows with black outline indicate abnormal chromosomes identified during karyotype analysis. Red, green, gold, and aqua arrow or arrow heads highlight positions of FISH signals corresponding to 5’-TMPRSS2, 3’-TMPRSS2, 5’-ERG and 3’-ERG.