Table 1.

Results of TMPRSS2 and ERG rearrangement with the novel 4-color FISH assay on castration resistant prostate cancer samples, including metastasis

Patient	Tissue	TMPRSS2:ERG fusion			Alternative rearrangement	CNI	Normal result	Concordance with RT-PCR/Gen-Probe
Patient	Tissue	Single (del)	Multiple (del)	Atypical	Alternative rearrangement	CNI	Normal result	Concordance with RT-PCR/Gen-Probe
9	Adrenal1	38%	56%					Y
9	Adrenal2	38%	52%					Y
9	Liver	64%	10%					Y
9	LN1	22%	44%					Y
9	LN2	30%	54%					Y
9	LN3	34%	24%					Y
9	LN4	32%	14%					Y
9	Lung1	32%	54%					Y
9	Lung2	22%	66%					Y
9	Spleen	40%	14%					Y
9	Prostate	32%	20%					Y
5	LN1	12%	80%					Y
5	LN2		100%					Y
5	LN3	20%	78%					Y
5	LN4	24%	72%					Y
5	LN5	12%	88%					Y
5	Prostate	84%	12%					Y
4	Liver	92%						Y
4	LN1	96%						N**
4	Lung1	96%						N**
4	Spleen	92%	8%					N**
2	LN1			88%				Y
2	LN2			97%				Y
2	LN3			100%				Y
2	Lung1			79%				Y
2	Lung2			88%				Y
2	Prostate			74%				Y
7	LN1				67%	20%		NA
7	LN2				89%			NA
7	LN3				84%			Y
7	LN4				97%			Y
7	Prostate				92%			Y
11	LN1					72%		Y
11	LN3					48%		Y
11	Prostate 1				78%			Y
11	Prostate 2				74%			Y
6	LN1					100%		Y
6	LN2					82%		Y
6	LN3					78%		Y
6	Peritoneal					92%		Y
1	Liver					12%		Y
1	LN1						Normal	Y
1	LN2						Normal	Y
1	Prostate					24%		Y
3	Liver					34%		Y
3	LN1					74%		Y
3	LN2					80%		Y
3	Lung					59%		Y
3	Prostate 1						Normal	Y
3	Prostate 2						Normal	Y
8	Liver						Normal	Y
8	LN1						Normal	Y
8	LN2						Normal	Y
8	LN3						Normal	Y
8	Lung						Normal	Y
8	Prostate						Normal	Y
LuCaP49^a	Omental fat	86%						N/Y
LuCaP86.2	Bladder	62%				16%		Y
LuCaP93^a	Prostate	60%	36%					Y
LuCaP145.1^a	Liver	94%						Y
LuCaP145.2^a	LN	14%	86%					Y
LuCaP35	LN			100%				Y
LuCaP35CR	LN			100%				Y
LuCaP92.1	Peritoneum			100%				Y
LuCaP58	LN				86%			Y
LuCaP96	Prostate				80%	26%		Y
LuCaP96CR	Prostate				98%	38%		Y
LuCaP146^b	NA				92%	28%		NA
LuCaP23.12	Liver					50%		N
LuCaP23.1CR	LN					60%		N
LuCaP69^b	NA					62%		NA
LuCaP70	Liver					64%		Y
LuCaP73	Prostate					60%		Y
LuCaP105	Rib					16%		Y
LuCaP115	LN					24%		Y
LuCaP141	Prostate					34%		Y
LuCaP77	Femur						Normal	Y
LuCaP78	Peritoneum						Normal	Y
LuCaP81	LN						Normal	Y
LuCaP136	Acites fluid (cells)						Normal	Y
LuCaP147	Liver						Normal	Y
LuCaP153^b	NA						Normal	NA

CR Castration resistant; these xenograft lines used to have the name suffix as “v” or “ai”

LN Lymph node

NA Data not available

Small cell carcinoma samples; discrepancy between FISH and RT-PCR in some (**) was documented previously (Saramäki et al., 2008). Therefore, fusion detected by FISH is considered as true positive.

Xenograft discontinued Blank indicates the corresponding FISH pattern was absent from the sample or below threshold