Skip to main content
. Author manuscript; available in PMC: 2013 Apr 22.
Published in final edited form as: Avian Dis. 2012 Dec;56(4):725–731. doi: 10.1637/10252-052212-Reg.1

Fig. 1.

Fig. 1

Schematic representation of rMDV expressing UL47-mRFP fusion proteins. Shown for each clone is the MDV genome depicting the locations of the terminal repeat long (TRL) and short (TRS), internal repeat long (IRL) and short (IRS), and unique long (UL) and short (US) regions. The position and orientation of the UL47 gene with respect to adjacent genes within the UL are shown. mRFP was fused to the C terminus of the UL47 tegument protein in three different BAC clones to generate rUL47-mRFP (A), rΔRLORF4-47mRFP (B), and rAU5-47mRFP (C) using the previously described BAC clones for rRB-1B (16), rRB-1B-ΔRLORF4 (20), and rAU5vTR (23). RLORF4 was deleted in both the TRL and IRL of the MDV genome in the rΔRLORF4-47mRFP clones, while both copies of the vTR template sequence (UCCCAAUC) was mutated to AUAUAUAU in rAU5-47mRFP. Only one copy within the TRL is shown for simplicity.