Fig. 1.

Western-blot with an anti-peptide QCG-16 for collagen IX in IL-1α treated cartilage explants. Media from days 0, 3, 6, 9, 12, 15, 18, 21 and 24 of IL-1α treated cartilage were separated by SDS-PAGE on 10% linear tricine gel and transferred to a polyvinylidene fluoride membrane. Position of collagen IX and its fragments was determined by using the NC4 domain antiserum. Produced fragments are shown by a, b, c, d and e related to their specific time releasing. The control gel was not shown here since no band was detected on this group gel.