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Western-blot with a polyclonal rabbit full length protein anti-bovine COMP (α-92) in IL-1α treated (A) and control (B) cartilage explants. Media from days 0, 3, 6, 9, 12, 15, 18, 21 and 24 of IL-1α treated cartilage were separated by SDS-PAGE on 10% linear tricine gel and transferred to a polyvinylidene fluoride membrane. Location of COMP monomers and its fragments were determined by using a polyclonal rabbit full length protein anti-bovine COMP (α-92).
