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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Cancer Discov. 2012 Nov 15;3(2):198–211. doi: 10.1158/2159-8290.CD-12-0353

Figure 3. Measurement of inter-kinetochore distance (IKD) in BUB1B resistant and sensitive cells.

Figure 3

(A) Cartoon showing IKD measurement.

(B) Measurement of IKDs in BTICs, NSCs, MEF, MEF-Ras, RPE and Hela cells using immunofluorescent staining of kinetochores. Constitutive associated centromere network (CCAN/CREST) proteins (red) and outer kinetochore protein, Hec1, (green) were visualized to identify kinetochore pairs. IKDs were measured between Hec1 centroids using Applied Precision Softworx software package.

(C–D) Quantification of IKDs from (B). *denotes p<.001 by student’s t-test.