(A) Cartoon showing IKD measurement.
(B) Measurement of IKDs in BTICs, NSCs, MEF, MEF-Ras, RPE and Hela cells using immunofluorescent staining of kinetochores. Constitutive associated centromere network (CCAN/CREST) proteins (red) and outer kinetochore protein, Hec1, (green) were visualized to identify kinetochore pairs. IKDs were measured between Hec1 centroids using Applied Precision Softworx software package.
(C–D) Quantification of IKDs from (B). *denotes p<.001 by student’s t-test.