Table 8.

Impact of sample quality on concordance between matched peripheral blood and bone marrow readouts.

Modulator	Modulator Time	Stain	Color	R2	R2 when difference in PH is included as a covariate	p (slope=0) for PH1	N
AraC+Daunorubicin	24 Hrs	cPARP	FITC-A	0.670	0.763	0.003	31
AraC+Daunorubicin	24 Hrs	p-Chk2	Alexa Fluor 647-A	0.845	0.845	0.864	31
Etoposide	24 Hrs	cPARP	FITC-A	0.669	0.683	0.241	35
Etoposide	24 Hrs	p-Chk2	Alexa Fluor 647-A	0.738	0.770	0.044	35
FLT-3 Ligand	15 min	p-Akt	Alexa Fluor 647-A	0.612	0.656	0.041	39
FLT-3 Ligand	15 min	p-ERK	PE-A	0.644	0.671	0.094	39
FLT-3 Ligand	15 min	p-S6	Alexa Fluor 488-A	0.725	0.785	0.003	39
G-CSF	15 min	p-STAT1	Alexa Fluor 488-A	0.574	0.668	0.003	39
G-CSF	15 min	p-STAT3	PE-A	0.777	0.848	0.000	39
G-CSF	15 min	p-STAT5	Alexa Fluor 647-A	0.777	0.844	0.000	39
Hydrogen Peroxide	15 min	p-Akt	Alexa Fluor 488-A	0.727	0.729	0.661	38
Hydrogen Peroxide	15 min	p-PLCγ2	PE-A	0.841	0.860	0.034	38
Hydrogen Peroxide	15 min	p-SLP76	Alexa Fluor 647-A	0.741	0.761	0.096	38
IL-27	15 min	p-STAT1	Alexa Fluor 488-A	0.857	0.880	0.012	39
IL-27	15 min	p-STAT3	PE-A	0.808	0.826	0.066	39
IL-27	15 min	p-STAT5	Alexa Fluor 647-A	0.734	0.744	0.248	39
PMA	15 min	p-CREB	PE-A	0.836	0.868	0.007	37
PMA	15 min	p-ERK	Alexa Fluor 647-A	0.768	0.837	0.001	37
PMA	15 min	p-S6	Alexa Fluor 488-A	0.806	0.842	0.009	37
SCF	15 min	p-Akt	Alexa Fluor 647-A	0.781	0.866	0.000	39
SCF	15 min	p-ERK	PE-A	0.397	0.445	0.087	39
SCF	15 min	p-S6	Alexa Fluor 488-A	0.723	0.829	0.000	39

¹The slope for the difference in PH between samples was found to be significant (P < 0.05, slope=0.0)