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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cytometry B Clin Cytom. 2012 Feb 14;82(3):158–172. doi: 10.1002/cyto.b.21007

Table 2.

Comparison of BMMC and PBMC measurements acquired on the flow cytometer using the Bland-Altman methodology.

Metric1 Measured Population Reference State Biology Investigated Total Nodes Nodes where measurements of BMMC and PBMC are
Equivalent3 Lin Equivalent4 Other5
ERF2 Calibrated Instrument Fluorescence Intensities None 41 15 (37%) 23 (56%) 3 (7%)
Basal = log2(ERFu/ERFa) Unmodulated Autofluorescence Basal Pathway Activity 16 15 (94%) 1 (6%) 0
log2 Fold = log2(ERFm/ERFu) Modulated Unmodulated Induced Pathway Activity 22 18 (82%) 3 (14%) 1 (5%)
Total Phospho = log2(ERFm/ERFa) Modulated Autofluorescence Total Pathway Activity 22 21 (95%) 1 (5%) 0
Ua Modulated Autofluorescence Basal Pathway Activity 38 22 (58%) 15 (39%) 1 (3%)
Uu Modulated Unmodulated Induced Pathway Activity 22 19 (86%) 3 (14%) 0
Total Node-Metrics 161 110 (68%) 46 (29%) 5 (3%)
1

The subscripts represent the following: a=auto fluorescence, m=modulated, u=unmodulated.

2

Equivalent Number of Reference Fluorophores

3

The variance does not depend on the size of the observations; either the difference between BMMC and PBMC was not statistically significant at the 5% level, or BMMC is a simple linear transformation of PBMC with R2 >= 80%.

4

For nodes that did not satisfy the requirements of the Bland-Altman method, Lin’s condordance correlation coefficient was applied to partition variation into precision (Pearson correlation) and accuracy(Cb). BM and PB were judged “Lin equivalent” if Pearson’s correlation >= 0.6 and Lin’s accuracy measure was >= 0.80 and the concordance correlation was at least 0.60.

5

Test for independence indicates standard deviation depends on the size of the observation and Lin’s concordance correlation coefficient was less than 0.60. Additional analysis is needed to verify the equivalence of BM and PB.