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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cytometry B Clin Cytom. 2012 Feb 14;82(3):158–172. doi: 10.1002/cyto.b.21007

Table 6.

Comparison of bone marrow and peripheral blood with respect to each node for the Ua1 metric comparing the modulated and auto fluorescence wells (No modulator)

Readout Simple linear regression
BM = intercept + slope*PB		 Bland-Altman Analysis
Difference3 (BM − PB)
Modulator Antibody Color R2 Slope p-value2 Intercept Bias estimate Std Dev p-value4 Classification5
No Modulator p-Akt Alexa Fluor 488-A 0.58 0.79 <.0001 0.13 0.004 0.044 0.5649 Equivalent
No Modulator p-Akt Alexa Fluor 647-A 0.74 0.93 <.0001 0.09 0.042 0.046 <.0001 Other
No Modulator p-CREB PE-A 0.65 0.76 <.0001 0.21 −0.006 0.043 0.3769 Equivalent
No Modulator p-ERK Alexa Fluor 647-A 0.76 0.85 <.0001 0.11 0.015 0.033 0.0049 Other
No Modulator p-ERK PE-A 0.63 0.61 <.0001 0.37 −0.005 0.035 0.3049 Other
No Modulator p-PLCγ2 PE-A 0.69 0.95 <.0001 0.03 −0.011 0.029 0.0201 Other
No Modulator p-S6 Alexa Fluor 488-A 0.47 0.63 <.0001 0.34 −0.01 0.043 0.1057 Equivalent
No Modulator (DMSO only) p-S6 Alexa Fluor 488-A 0.58 1.01 <.0001 −0.02 −0.013 0.032 0.0085 Other
No Modulator p-SLP-76 Alexa Fluor 647-A 0.77 0.88 <.0001 0.1 0.02 0.038 0.0012 Other
No Modulator p-STAT1 Alexa Fluor 488-A 0.71 0.88 <.0001 0.06 −0.031 0.068 0.0037 Other
No Modulator p-STAT3 PE-A 0.61 0.77 <.0001 0.19 −0.013 0.051 0.0908 Equivalent
No Modulator p-STAT5 Alexa Fluor 647-A 0.73 0.82 <.0001 0.17 0.027 0.049 0.0005 Other
1

Ua=Mann-Whitney U comparing modulated and auto fluorescence wells (No modulator)

2

p-value of the test that the slope equals 0.

3

When (BM-PB) and (BM+PB)/2 are not correlated, the difference between the BM and PB mean values is an unbiased estimate of the bias, the difference one would expect on average between BM and PB flow readouts.

4

When the p-value is marked in dark green, the bias is 0 and BM may be substituted for PB and vice-versa. When the p-value is marked in light green, the substition can be made with the addition (subtraction) of the bias.

5

Classification of the comparison of BM and PB for each nodemetric following the method of Altman and Bland (1983). Nodemetrics where the BM and PB values are judged equivalent are classified as “Equivalent” and can be used interchangeably on average. Nodemetrics classified as “Other” require further investigation before they can be used interchangeably.