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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cytometry B Clin Cytom. 2012 Feb 14;82(3):158–172. doi: 10.1002/cyto.b.21007

Table 7.

Comparison of bone marrow and peripheral blood with respect to each node for the Uu1 metric comparing the modulated and auto fluorescence wells

Readout Simple linear regression
BM = intercept + slope*PB		 Bland-Altman Analysis
Difference3 (BM − PB)
Biological Effect Modulator Antibody Color R2 Slope p-value2 Intercept Bias estimate Std Dev p-value4 Classification5
Apoptosis
Dauno + Ara-C Cleaved PARP FITC-A 0.685 0.84 <.0001 0.11 0.002 0.083 0.8783 Equivalent
Dauno + Ara-C p-Chk2 Alexa Fluor 647-A 0.829 0.82 <.0001 0.11 −0.003 0.052 0.7456 Equivalent
Etoposide Cleaved PARP FITC-A 0.661 1 <.0001 0.02 0.014 0.041 0.0412 Other
Etoposide p-Chk2 Alexa Fluor 647-A 0.737 0.74 <.0001 0.19 0.014 0.057 0.1224 Equivalent
Signaling
FLT-3 Ligand p-Akt Alexa Fluor 647-A 0.622 0.77 <.0001 0.13 0.006 0.035 0.2373 Equivalent
FLT-3 Ligand p-ERK PE-A 0.651 0.72 <.0001 0.16 0.004 0.049 0.5902 Equivalent
FLT-3 Ligand p-S6 Alexa Fluor 488-A 0.704 0.8 <.0001 0.14 0.004 0.069 0.7139 Equivalent
G-CSF p-STAT1 Alexa Fluor 488-A 0.568 0.84 <.0001 0.1 0.015 0.062 0.1119 Equivalent
G-CSF p-STAT3 PE-A 0.74 0.86 <.0001 0.11 0.021 0.073 0.0605 Equivalent
G-CSF p-STAT5 Alexa Fluor 647-A 0.746 0.86 <.0001 0.12 0.024 0.073 0.029 Other
Hydrogen Peroxide p-Akt Alexa Fluor 488-A 0.681 0.83 <.0001 0.12 −0.013 0.054 0.1236 Equivalent
Hydrogen Peroxide p-PLCγ2 PE-A 0.854 0.93 <.0001 0.07 0.032 0.078 0.008 Biased Low
Hydrogen Peroxide p-SLP-76 Alexa Fluor 647-A 0.687 0.93 <.0001 0.04 0.011 0.069 0.2816 Equivalent
IL-27 p-STAT1 Alexa Fluor 488-A 0.827 0.9 <.0001 0.07 −0.001 0.06 0.8822 Equivalent
IL-27 p-STAT3 PE-A 0.756 0.85 <.0001 0.09 0 0.061 0.9904 Equivalent
IL-27 p-STAT5 Alexa Fluor 647-A 0.627 0.78 <.0001 0.12 −0.004 0.06 0.6927 Equivalent
PMA p-CREB PE-A 0.763 0.84 <.0001 0.13 0.021 0.073 0.0663 Equivalent
PMA p-ERK Alexa Fluor 647-A 0.716 0.85 <.0001 0.12 −0.002 0.069 0.887 Equivalent
PMA p-S6 Alexa Fluor 488-A 0.742 0.8 <.0001 0.16 0.008 0.067 0.4246 Equivalent
SCF p-Akt Alexa Fluor 647-A 0.726 0.84 <.0001 0.12 0.026 0.054 0.0017 Other
SCF p-ERK PE-A 0.434 0.59 <.0001 0.23 0.008 0.054 0.3389 Equivalent
SCF p-S6 Alexa Fluor 488-A 0.68 0.74 <.0001 0.18 0.013 0.078 0.2677 Equivalent
1

Uu=Mann-Whitney U comparing modulated and unmodulated wells

2

P-value of the test that the slope equals 0.

3

When (BM−PB) and (BM+PB)/2 are not correlated, the difference between the BM and PB mean values is an unbiased estimate of the bias, the difference one would expect on average between BM and PB flow readouts.

4

When the p-value is marked in dark green, the bias is 0 and BM may be substituted for PB and vice-versa. When the p-value is marked in light green, the substition can be made with the addition (subtraction) of the bias.

5

Classification of the comparison of BM and PB for each nodemetric following the method of Altman and Bland (1983). Nodemetrics where the BM and PB values are judged equivalent are classified as “Equivalent” and can be used interchangeably on average. Nodemetrics classified as “Other” require further investigation before they can be used interchangeably.