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RT-PCR analysis of mRNA transcripts for NGF (top panel) and its p75NTR (mid panel) and trkA (bottom panel) receptors in lung tissues from a pathogen-free rat exposed to nicotine for 28 days using a subcutaneous osmotic mini-infusion pump (right bands) versus its control exposed to vehicle with the same technique (left bands). The representative electrophoresis on ethidium bromide-stained agarose gel shows increased NGF and p75NTR expression without change in trkA.
